Contribution of Supercritical Fluid Chromatography coupled to High Resolution Mass Spectrometry and UV detections for the analysis of a complex vegetable oil - Application for characterization of a Kniphofia uvaria extract

摘要

Lipids are an important and diverse group of naturally occurring organic compounds present in all biological entities. They play many essential roles in the survival of the organisms. Analysis of lipids is extremely complex due to their large variety of chemistries. In this work, we describe an innovative method for the characterization of the lipid composition in a complex and unknown vegetable oil: an extract of Kniphofia uvaria seeds; using Supercritical Fluid Chromatography (SFC) coupled to Atmospheric Pressure Chemical Ionization (APCI), High-Resolution Mass Spectrometry (HRMS) and UV detections. Thanks to the properties of supercritical CO2 allowing the use of a large number of chromatographic columns, an effective separation for this complex matrix was obtained. (C) 2016 Published by Elsevier Masson SAS on behalf of Academie des sciences.