Protein hynamics studies on a wheat type 2 lipid transfer protein

摘要

Plant non specific lipid transfer proteins form a superfamily of related proteins composed of type 1 and type 2 LTPs.Type 1 LTPs have been less extensively studied than type 1 and differ in term of primary sequence,molecular mass and transfer efficiency.We have undertaken NMR studies of the wheat 7kDt type 2 LTP.Whilst the 3D structure determination is inprogress,we have studied the protein hynamics.Two zones hae been defined within the protein.One remains unperturbed,the LTP being liganded or not;the other one exists in different states,depending on the ligandation,and on the kind of lipid interacting with the protein.S~2 determinatiion showed a rigid backbone.Multifield analysis allowed the calculation of the chemical exchange rate for each residue.