MATCH-BOX-SERVER - A MULTIPLE SEQUENCE ALIGNMENT TOOL PLACING EMPHASIS ON RELIABILITY

摘要

Motivation: The Match-Box software comprises protein sequence alignment tools based on strict statistical thresholds of similarity between protein segments. The method circum vents the gap penalty requirement: gaps being the result of the alignment and not a governing parameter of the procedure. The reliable conserved regions outlined by Match-Box are particularly relevant for homology modelling of protein structures, prediction of essential residues for site-directed mutagenesis and oligonucleotide design for cloning homologous genes by polymerase chain reaction (PCR).