OPTICAL PROPERTIES OF NORMAL AND THERMALLY COAGULATED CHICKEN LIVER TISSUE MEASURED ex vivo WITH DIFFUSE REFLECTANCE

摘要

The purpose of the present study is to determine the optical properties of normal and thermally coagulated chicken liver at 720, 740, 770, 810, 825 and 840 nm wavelengths of laser irradiation. So, we were able to eval-uate these optical properties (absorption and scattering coefficients) with ex-vivo study using Kubelka–Munk model (KMM) from the radial dependence of the diffuse reflectance with femtosecond pulsed laser in near I R region. These coefficients were significantly increased with coagulation. The penetration depths of the dif-fused light have been reported to a maximum value of 8.12 ± 0.36 mm in normal liver and 2.49 ± 0.17 mm in coagulated liver at 840 nm showing increasing behavior towards IR region. The Monte Carlo simulation was used to check the theoretical validation of measured optical properties of the tissue that showed a good match with our experimental results. We believe that these differences in optical properties will be help-ful for the understanding and optimal use of laser applications in medicine and differential diagnosis of tissues by using different optical methods. Especially for the investigation of biological tissue for photo-dynamic therapy (PDT), the knowledge of the specific optical properties and their thermo-induced changes is important.