Development and approbation of test systems for the determination of herpes simplex virus and cytomegalovirus antigens by the method of lanthanide immunofluorescent assay

摘要

On the basis of the lanthanide immunofluorescent assay (UFA) test systems for the determination of herpes simplex virus (HSV-LIFA) and cytomegalovirus (CMV-UFA) antigens have been developed. The test system HSV-LIFA includes the sandwich of monoclonal antibodies (McAb) HSV A.3.3. or B-11 to type 2 HSV, strain BH; the test system CMV-UFA includes the sandwich of rabbit McAb to CMV, strain AD 169. The approbation of the test systems has revealed that they insure the specific detection of HSV and CMV antigens in clinical specimens (urine, blood, liquor, saliva), the UFA results well correlating with the data on the isolation of viruses in cell cultures, with the results obtained by other diagnostic methods and with clinical manifestations of diseases. UFA has been shown to be more sensitive than the enzyme immunoassay.