Modified and improved method for the measurement of plasma acylcarnitines by online extraction coupled to liquid chromatography-tandem mass spectrometry without derivatization.

摘要

Tandem mass spectrometry (MS/MS) is being increasingly used to identify and measure acylcarnitines in blood and urine of children suspected of having fatty acid oxidation disorders and other inborn errors of metabolism [1-7]. Rapid MS/MS analysis requires simple and efficient sample preparation. Recently, we have developed a LC-MS/ MS method for the online extraction of acylcarnitines in plasma without derivatization that requires precipitation of proteins with acetonitrile as the only sample treatment step .