New approaches to construction of transgenic animals with a high-level tissue-specific expression of foreign genes: construction and reconstruction of genomic domains

摘要

The major problems in constructing transgenic animals and approaches to their solution are discussed. Modern views of the structural-functional organization of the eukaryotic genome are considered. The domain hypothesis of the genome organization is analyzed in detail, as many problems associated with a ow level and a lack of tissue specificity of transgene expression can be solved at the step of construction (or reconstruction of existing domains).