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Fishery by-product as a nutrient source for bacteria and archaea growth media

机译:渔业副产品作为细菌和古细菌生长培养基的营养源

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摘要

A highly soluble fish protein hydrolysates (FPH) with an 80% protein (peptide size between 1.5 and 20 kDa) and a low free amino acid content was obtained from hake (Merluccius hubssi) filleting waste [Lat. Am. Appl. Res. 30 (2000) 241]. Assays with Halobacterium salinarum, Escherichia coli, Bacillus subtilis and Staphylococcus epidermidis were performed in order to test that FPH as nutrient source for archaea and eubacteria culture media. Cell growth was evaluated by plate count, and by monitoring turbidity and nucleic acids content in liquid cultures. Neither cell growth nor generation times resulting from control and FPH cultures exhibited statistically significant differences at alpha: 0.05 suggesting that FPH can be used as an alternative substrate for microorganism cultural purposes. (C) 2004 Elsevier Ltd. All rights reserved.
机译:从无须鳕(Merluccius hubssi)去骨切片废料中获得了一种高可溶性鱼蛋白水解物(FPH),其蛋白含量为80%(肽大小在1.5至20 kDa之间),游离氨基酸含量较低。上午。应用Res。 30(2000)241]。为了测试FPH作为古细菌和真细菌培养基的营养来源,使用了盐杆菌,大肠杆菌,枯草芽孢杆菌和表皮葡萄球菌进行了测定。通过平板计数,并通过监测液体培养物中的浊度和核酸含量来评估细胞生长。对照和FPH培养产生的细胞生长和生成时间均未在α:0.05处显示出统计学上的显着差异,这表明FPH可用作微生物培养目的的替代底物。 (C)2004 Elsevier Ltd.保留所有权利。

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