Biohydrogen production in alkalithermophilic conditions: Thermobrachium celere as a case study

摘要

In the present work the hydrogenesis in the anaerobic alkalithermophilic bacterium Thermobrachium celere was studied. The impact of several factors on hydrogen production during glucose fermentation was investigated in batch conditions. The optimal hydrogen production occurred at pH_(67°C) 8.2 with phosphate buffer concentration of 50mM. Hydrogen yield reached the highest value of 3.36mol H_2/mol glucose when the partial pressure in the gas headspace was reduced. Supplementation of nitrogen sources and iron affected hydrogen production. Under optimized conditions, the maximum H_2 accumulation and H_2 production rate were estimated to be respectively 124.3mmol H_2/l culture and 20.7mmol H_2/l/h. Considering the efficient and rapid hydrogen evolution, and the ability to grow in extreme environments, T. celere might be a good candidate for biohydrogen production in open (non-sterile) bioprocess system.