Plant Regeneration from Embryogenic Calli of the Wild Sugarcane (Saccharum spontaneum L.) Clone 'Glagah Kloet'

摘要

A wild sugarcane clone, Saccharum spontaneum 'Glagah Kloet', is utilized as breeding material for development of high-yielding sugarcane cultivars. In the present study, we established a plant regeneration system for this clone to increase its potential for use in molecular breeding of sugarcane with a transformation system in the future. Although embryogenic callus was not induced with the medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) alone that has been used commonly for callus inductionof sugarcane, we obtained embryogenic calli when apical meristems aseptically isolated from shoots were cultured on callus induction medium containing both 0.01mg L~(-1) benzyladenine (BA) and 5 mg L~(-1)2,4"D- This finding indicates that BA is positively effective for induction of embryogenic calli of 'Glagah Kloet'. The embryogenic calli produced shoots when cultured on medium containing 0.3 mg L~(-1)gibberellic acid or on hormone-free medium supplemented with 3 g L~(-1)activated charcoal (AC). The morphology of the shoots clearly differed between the regeneration media. The shoots formed on medium containing gibberellic acid were finer and softer, and the leaf color was lighter compared with those formed on the hormone-free medium containing AC, thus more healthy shoots were regenerated.