A chromogranin A ELISA absent of an apparent high-dose hook effect observed in other chromogranin A ELISAs

摘要

Background: Routine testing for chromogranin A (CgA) using an established commercial ELISA revealed an apparent high-dose hook effect in approximately 15% of specimens. Investigations found the same effect in two additional ELISAs. We hypothesized that a CgA derived peptide(s) at high concentrations was responsible but experiments were inconclusive. Here we describe the analytical performance characteristics of the Chromoa (TM) CgA ELISA that did not display the apparent high-dose hook effect.