オプトジェネティクスを用いた覚醒マウス用無線小型光刺激装置の開発

摘要

I produced a miniaturized, multicode, multiband, and programmable light-emitting diode (LED)-stimulator for the wireless control of optogenetic experiments. The LED-stimulator can drive three independent LEDs upon reception of an infrared (IR) signal from a newly developed IR-transmitter. Individual LED photopulse patterns are assigned to as many as 256 different codes of IR signals generated by the IR-transmitter. Using these devices, I remotely controlled the moving direction of a Thyl-ChR2-YFP transgenic mouse by transcranially illuminating the corresponding hemisphere of the primary motor cortex. The IR-transmitter and LED-stimulator will be particularly useful in experiments where free movements or patterned concurrent stimulations are desired, such as testing the social communication of rodents. I summarized optogenetics and available wireless photo-stimulators in this review.