Daily Intake of Probiotics with High IFN-y/IL-10 Ratio Increases the Cytotoxicity of Human Natural Killer Cells: A Personalized Probiotic Approach

摘要

A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-y/IL-10 or IL-10/IFN-y among six probiotics, including L paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L rhamnosus AD500, and L. reuteri BR101. One hundred volunteers were included and their PBMCs were collected and stimulated by the six probiotics. People who belonged to the IFN-y group took the probiotics that exerted the highest ratio of IFN-y/IL-10 and vice versa in IL-10 group. A significant increase in NK cytotoxicity of 69 volunteers in the IFN-y group was observed compared to the IL-10 group (? = 21) and control group (n = 10). The result also showed that L. paracasei BRAP01 and L acidophilus AD300 were the two dominant inducers in IFN-y group which yielded higher value of IFN-y/IL-10 than the other 4 probiotics, while L reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-y in the IL-10 group. Our finding highlighted the concept of personalized probiotics and also provided a good foundation to investigate the probiotics with NK activity.