Elecrophysiological Evaluation for Conditions of Preservation of Isolated Human Colon Tissues

摘要

The aim of the present study was to examine conditions for preserving isolated human colon tissues when assesing colonic drug absorptionl Isolated human colon tissues wer kept in various conditions. And electrophysiologicla analysis at 37deg C was then carried out to assess the integrity of the paracellular and transcellular route using Ussing-type chambers. the membrane resitance (Rm) of isolated human colon preserved at 4deg C for 3,6 or 9 hours increased up to 90 min and was thereafter constant for at least 40 min. The permeability of FD-4, a paracellular permeable compound, was restricted at constant Rm. On the other hand, the value of potntial diference and of short circuit current as well as that of Rm was constant for at leeast 40 min. Neither preservation for 12 hours at 4deg C nor that for 3 hours at room temperatue was suitable when evaluated in terms of the recovery fo Rm. These results indicate that cell viability was guaranteed for 9 hours provided that isolated human colon tissues were preserved at 4deg C. Furthermore, it is neccessary to preincubate for at least 90 min to obtain a structural and functional recovery of the paracellular and transcellular route.