PLGA Microsphere Construct Coated with TGF-beta 3 Loaded Nanoparticles for Neocartilage Formation

摘要

Polymeric microsphere system has been widely used in tissue-regeneration matrix and drug delivery systems.To apply these biomaterials as novel cell supporting matrix for stem cell delivery,we have devised a novel method for the fabrication of nanostractured 3D scaffolds that growth factor loaded heparin/poly(L-lysine)nanoparticles were physically attached on the positively charged surface of PLGA microspheres precoated with low molecular weight of poly(ethyleneimmine)(PEI)via a layer-by-layer(LbL)system.Based on a previous study,we have prepared poly(lactide-co-glycolide)(PLGA)microspheres harboring heparin/poly(L-lysine)loaded with growth factors.Growth factor loaded heparin/poly(L-lysine)nanoparticles,which were simply produced as polyion complex micelles(PICM)with diameters of 50-150 nm,were fabricated in the first step.Microsphere matrix(size,20~80 nm)containing TGF-beta 3 showed a significantly higher number of specific lacunae phenotypes at the end of the 4 week study in vitro culture of mesenchymal stem cells.Thus,growth factor delivery of PLGA microsphere can be used to engineer synthetic extracellular matrix.This PLGA microsphere matrix containing TGF-beta 3 showed promise as coatings for implantable biomedical devices to improve biocompatibility and ensure in vivo performance.