Heterostereocomplexes prepared from D-poly(lactide) and leuprolide.I.Characterization

摘要

Heterostereocomplexes between D-PLA and L-peptides,obtained by spontaneous precipitation from acetonitrile solution,were characterized by thermal analysis and microscopic techniques.Differential scanning calorimetry showed two transition endotherms,one for the alpha form that melt at 178 degC and one for the beta form of PLA that melts at 169 degC.A linear correlation was found between the enthalpy of both melt temperatures and the peptide concentration.The complexation was monitored by a change in morphology,which was imaged by AFM-tapping mode.The initial fibrous network of D-PLA changed to uniform disks of 100 nm in diameter and 2.5 nm in height of the heterostereocomplex.Rhodamine B labeled leuprolide was complexed selectively to D-PLA,which was chemically bound onto mica plates.Addition of L-PLA to the complex enabled displacement of the peptide,which was observed by fluorescent spectrometry and confocal microscopy.These results provide a method,which enables one to obtain an expression for the relative interaction strength between various stereoselective polymers and polypeptides with opposite enantiomeric configuration.