Remodeling of the actin cytoskeleton of cultivated human endothelium cells during clinostatting

摘要

Architectonics of the fibrillar actin in cultures of human endothelium cells (EC) during and after extended (144 hrs.) clinostatting was examined under the fluorescent microscope. In control EC cultures, F-actin was composed of a network of filaments located both in the center and along the periphery of cells. One hour of rotation did not visibly affect the actin skeleton, however, in two hours there were cells with filaments displaced toward the membrane. Twenty-four hours of rotation caused total remodeling of the cytoskeleton so that practically the whole population of thin filaments had migrated toward the periphery. In most of the cells the intercell contact area formed undulating edges rich in F-actin. A similar picture could be seen after 144 hrs. of rotation. The actin skeleton partially recovered in 4 hrs. and regained the normal structure in 24 hrs. of rehabilitation. Same period was required for cells to restore their structure completely following the extended clinostatting. These data suggest sensitivity of the EC cytoskeleton to changes in the gravity vector. Remodeling of the actin skeleton in a changed gravity is reversible and proceeds more rapidly than recovery. kletok cheloveka v usloviiakh klinostatirovaniia. environmental medicine.