首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >Dramatic Changes in 67 miRNAs During Initiation of First Wave of Spermatogenesis in Mus musculus Testis: Global Regulatory Insights Generated by miRNA-mRNA Network Analysis
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Dramatic Changes in 67 miRNAs During Initiation of First Wave of Spermatogenesis in Mus musculus Testis: Global Regulatory Insights Generated by miRNA-mRNA Network Analysis

机译:在小家鼠睾丸精子发生的第一波启动过程中67个miRNA的剧烈变化:由miRNA-mRNA网络分析产生的全球监管见解

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We mapped global changes in miRNA and mRNA profiles spanning the first wave of spermatogenesis using prepubertal (Postnatal Day 8 [P8]), pubertai (P16), and adolescent (P24) Mus musculus testes and identified the differential expression of 67 miRNAs and 8226 mRNAs. These two data sets were integrated into miRNA-deperident regulatory networks based on miRWalk predictions. In a network representing the P8 to P16 transition, downregulation of four miRNAs and upregulation of 19 miRNAs were linked with 81 upregulated target mRNAs and 228 downregulated target mRNAs, respectively. Furthermore, during the P16 to P24 transition, two miRNAs were downregulated, and eight miRNAs were upregulated, which linked with 64 upregulated mRNAs and 389 downregulated mRNAs, respectively. Only three of the miRNAs present in the network (miR-34b-5p, miR-34c, and miR-449a) showed a progressive increase from P8 through P16 to P24, while the remaining miRNAs in the network showed statistically significant changes in their levels either during the P8 to P16 transition or during the P16 to P24 transition. Analysis of the chromosomal location of these differentially expressed miRNAs showed that 14 out of 25 miRNAs upregulated from P8 to P16, and 18 out of 40 miRNAs upregulated from P8 to P24 were X-linked. This is suggestive of their escape from meiotic sex chromosome inactivation and postmeiotic sex chromatin. This integrated network of miRNA-level and mRNA-level changes in mouse testis during the first wave of spermatogenesis is expected to build a base for evaluating the role of miRNA-mediated gene expression regulation in maturing mammalian testis.
机译:我们绘制了使用青春期前(产后第8天[P8]),青春期(P16)和青春期(P24)小家鼠睾丸的精子发生第一波的miRNA和mRNA概况的全球变化,并鉴定了67个miRNA和8226个mRNA的差异表达。根据miRWalk预测,将这两个数据集整合到miRNA依赖的监管网络中。在代表P8到P16过渡的网络中,四个miRNA的下调和19个miRNA的上调分别与81个上调的目标mRNA和228个下调的目标mRNA相关。此外,在从P16到P24的过渡过程中,两个miRNA被下调,八个miRNA被上调,分别与64个上调的mRNA和389个下调的mRNA相关。网络中仅存在的三个miRNA(miR-34b-5p,miR-34c和miR-449a)显示从P8到P16逐渐增加到P24,而网络中的其余miRNA的水平显示出统计学上的显着变化在P8到P16转换期间或在P16到P24转换期间。这些差异表达的miRNA的染色体位置分析表明,从P8到P16上调的25个miRNA中有14个,从P8到P24上调的40个miRNA中有18个是X连锁的。这表明他们摆脱了减数分裂性染色体失活和减数分裂后的性染色质。在精子发生的第一波过程中,小鼠睾丸中miRNA水平和mRNA水平变化的这种集成网络有望为评估miRNA介导的基因表达调控在成熟的哺乳动物睾丸中的作用奠定基础。

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