Effects of contrast media on the erythrocyte mobility from sedimentation rate at different osmotic pressures

摘要

X-ray contrast media (CMs) influence various systems in blood circulation. Mobility of erythrocyte μ has been determined from the erythrocyte sedimentation rate s at different osmotic pressure π, to compare effects of ionic CM to those of non-ionic CM. Tow kinds of CMs, ionic Urografin 76% (URO) and non-ionic Iopamiron 300 (IOP), were employed. The density ρ_c and the volume V of erythrocyte, and s were measured at 25 ℃ by using a vibrating densimeter and the Coulter counter, and the Westergren tube, respectively. Swine erythrocytes were suspended in saline solutions without CMs (SAL) and with CMs at various π, where the hematocrit was ca. 10% and the CM concentrations were ca. 6.7 wt.%. ρ_c and V behaved as a single function of π both without CMs and with CMs, meaning RBC to be a micro-osmometer. While μ increased with V vigorously for SAL at the highest level, μ varied slightly for URO at the lowest level and moderately for IOP. Deformability of erythrocyte may be decreased more seriously for ionic CM than for non-ionic CM at the physiological condition of π, which may disturb blood microcirculation.