Response to: On methods of determination of homogeneous nucleation rates of protein crystals

摘要

In their letter, Galkin and Vekilov suggest that our analysis [Dixit et al., Colloids Surf. A 190, 47 (2001)] of their experimental technique [Galkin and Vekilov, J. Phys. Chem. B 103, 10965 (1999); J. Am. Chem. Soc. 122, 156 (2000)] for estimating protein crystal nucleation rates contains inconsistencies. Here, we examine their criticisms and find that our analysis does not contain inconsistencies. We conclude by restating our claim in [Dixit et al., Colloids Surf. A 190, 47 (2001)] that Galkin and Vekilov underestimate protein crystal nucleation rates by several orders of magnitude because of the way their experimental data is interpreted. (C) 2002 Elsevier Science B.V. All rights reserved. [References: 12]