Comparative insecticide susceptibility and detoxification enzyme activities among pestiferous Blattodea

摘要

Topical bioassays using propoxur, chlorpyrifos, and #lambda#-cyhalothrin were conducted on eight cockroach species. Based on lethal dose values, the relative toxicities of the insecticide classes were generally pyrethroid > carbamate > organophosphorous. #lambda#-Cyhalothrinand propoxur were more toxic toward the Blattidae as compared with the Blattellidae. The order of #lambda#-cyhalothrin toxicity was Periplaneta americana > Periplaneta brunnea = Periplaneta australasiae = periplaneta fuliginosa = Blatta orientalis > Blattella asahinai = Blattella germanica > Blattella vaga. The order of propoxur toxicity was B. orientalis > p. americana > P brunnea = p. australasiae > B. asahinai > p. fuliginosa = B. germanica > B. vaga. The order of chlorpyrifos toxicity was p. americana > B. asahinai =B. vaga > B. orientalis = P. australasiae = p. brunnea > B. germanica = P. fuliginosa. Detoxification enzyme activities for each species also were measured and compared with insecticide toxicity. Propoxur LD_(50) was significantly (P = 0.01; r = 0.81) correlated with glutathione S-transferase activity. #lambda#-Cyhalothrin LD_(50) correlated with methoxyresorufin O-demethylase activity (p = 0.01; r = 0.81), carboxylesterase activity (p = 0.03; r = -0.75), general esterase activity (p = 0.02; r = -0.79), and cockroach weight (p=0.01; r = -0.95).