Molecular characterization of hypoxia inducible factor-1 (HIF-1) from the white shrimp Litopenaeus vannamei and tissue-specific expression under hypoxia

摘要

Hypoxia inducible factor 1 (HIF-1) is a key transcription factor that regulates a variety of molecular responses tohypoxia. Some marine crustaceans experience changes of oxygen tension in their aquatic environment, butknowledge about the function and expression of HIF-1 is very limited. HIF-1 is a heterodimer composed by a and psubunits. We report the complete cDNA sequences of HIF-1α and HIF-1β from the white shrimpLitopenaeusvannamei. HIF-la (LvHIF-1a) is 3672 bp and codes for 1050 amino acids, while HIF-1β is 2135 bp (LvHIF-10) and 608amino acids. Both, the α and β subunits have the helix-loop-helix (bHLH) and PAS domains. HIF-la also has theoxygen dependent degradation (ODD) and the C-terminal transactivation domain (C-TAD), important for regulationin normoxia. Phylogenetic analyses of the proteins indicate separation of invertebrates from vertebrates. Largedifferences of HIF-lα and HIF-1β transcripts abundance were detected in gills, hepatopancreas and muscle undernormoxia (6 mg/L dissolved oxygen, DO) and hypoxia (2.5 and 13 mg/L DO). HIF-la was more abundant in gillsand HIF-1β in hepatopancreas. Large changes in response to hypoxia were detected for H1F-lα in gills, while HIF-1βremained fairly constant Glucose and lactate in hemolymph increased rapidly in hypoxia in all cases and up to 4.7and 5.0-fold, respectively, in response to 1.5 mg/L DO for 1h.