Effects of environmentally relevant concentrations of nonylphenol on growth and 20-hydroxyecdysone levels in mysid crustacean, Americamysis bahia

摘要

We investigated the effects of environmentally relevant concentrations of nonylphenol (NP) on survival,growth, sexual development and molting of the mysid Americamysis bahia (Crustacea: Mysidacea), includingassay of 20-hydroxyecdysone (20E) levels. Seven-day-old mysids were exposed to NP concentrations of 03, 1,3, 10 and 30 pg/L for 14 days. Among growth-related traits we assayed, an effect on body length was the mostprominent, with the effects observed at concentrations as low as 1 pg/L NP. In addition, the total number ofmolting exposed to NP for 14 days was significantly decreased in the treatment groups at 10 or 30 pg/Lrelative to the controls. These results clearly indicate that subchronic exposure to environmentally relevantconcentrations of NP affects growth of mysids. Furthermore, we investigated the effect of NP on production of20E in female mysids during the molting period. Production of 20E in pre-molting mysids exposed to 30 pg/Lof NP was lower than those in control groups, and exposure of female mysids to NP disrupted molting cycles.Taken together, the results suggest that environmentally relevant concentrations of NP can disrupt growth ofA. bahia via inhibition of 20E levels and an associated delay in molting.