Production of Streptoverticillium cinnamoneum transglutaminase and cinnamic acid by recombinant Streptomyces lividans cultured on biomass-derived carbon sources

摘要

Transglutaminase from Streptoverticillium cinnamoneum (StvcMTG) was produced using recombinant Streptomyces lividans. When grown on glycerol and xylose as sole carbon sources, S. lividans/StvcMTG produced 360 and 530. mg of StvcMTG per liter, respectively. With starch and xylan, the strain produced 230 and 400. mg of StvcMTG per liter, respectively. Recombinant S. lividans/. encP, which expresses phenylalanine ammonia lyase from Streptomyces maritimus, produced 160. mg/L of cinnamic acid from cellulose. These results show that S. lividans can assimilate various carbon sources and produce useful compounds in desirable quantities.