Protection of Pacific White Shrimp, Litopenaeus vannamei against White Spot Virus Following Administration of N-terminus Truncated Recombinant VP28 Protein Expressed in Gram-positive Bacteria, Brevibacillus choshinensis

摘要

To increase survival of different commercially important shrimp species against white spot virus disease (WSVD), a number of combinations using different recombinant VP28 protein forms expressed in Gram-negative or -positive bacteria have been tested.Here, we explored yet another combination to maximize utilization of VP28 specific to Pacific white shrimp, Litopenaeus vannamei to protect it against WSV. Large amounts of N- and an N+C-terminus-deleted recombinant VP28 (rN-VP28 and rNC-VP28) were produced in large amounts in a Gram-positive bacterium, Brevibacillus choshinensis and then administered to the shrimp by either oral feeding or intramuscular injection. Feeding rN-VP28 (50 mu g day~(-1) for 7 days), but not rNC-VP28, provided each shrimp significant protection against WSV. Furthermore, we showed that both oral delivery and intramuscular injection of rN-VP28 were effective for Pacific white shrimp.