Hatching Inhibition Test Using the Japanese Whiting Sillago japonica as an Acute Toxicity Test for Marine Fish Species

摘要

Hatching inhibition tests of the Japanese whiting Sillago japonica were undertaken as toxicity tests that used marine fish species. The fertilized eggs were exposed to several chemicals within 4 h after spawning. After 24 h, we observed the eggs for hatching, and the median-effective concentrations (ECso) were calculated based on the normal hatching rates. Organic solvents such as dimethyl sulfoxide, N,N-dimethylformamide, and ethanol had low toxic effects on the hatching of this species. Toxicity tests using pentachlorophenol and sodium dodecyl sulfate were conducted several times, and the toxicity values obtained for each chemical were observed to remain constant between tests.