Change in the nuclear phase and kinase activity during in vitro maturation of the porcine oocytes

摘要

It aimed at investigating a relation with maturity of the cytoplasm which made the index the maturation promoting factor (MPF) and mutagens activated protein (MAP) kinase activity which participate in nuclear and the cell of the porcine oocytcs. Changes in the nuclear phase of porcine oocytes were examined in 4 time intervals during maturation, beginning with the in vitro culture stage and ending 48 hours after cultivation. We also examined the activity of histone H1 kinase (H1k) and MAP kinase (MAPk) which are markers for the MPF and their correlation to the nuclear phase transition and culture time. Since activity might change delicately when many ova are required to measure kinase activity and the sample changed, the activity of both kinase of the same sample was measured at once.