Superoxide dismutase-like activity of boar semen and effect of SOD on the preservation of boar spermatozoa

摘要

The purpose of this experiment was to evaluate the superoxide dismutase (SOD)-like activity of boar seminal plasma, the change of SOD-like activity in the semen stored in Modena extender during preservation and to clarify the effect of addition of SOD to Modena extender on the motility of boar spermatozoa. The SOD-like activity was measured by Electron Spin Resonance Spectroscopy. The SOD-like activities of semina plasma of boar A and B were 9.7+-0.9#mu#/ml and 6.1+-0.2#mu#/ml, respectively. The SOD-like activity in the semen diluted with Modena extender did not change during the storage peiod of 28 days at 10 deg C. The addition of SOD to the extended semen improved sperm viability during storage of 28 days and the best result was obtained from the spermatozoa suspended in Modena extender added 800#mu#/ml of SOD. The results suggest that the addition of SOD to Modena extender is useful for long-term maintenance of good motility in liquid storage of boar spermatozoa.