Development of Duplex SYBR Green Real-Time PCR for Rapid and Simultaneous Detection of16 Specific Genes of16 Major Foodborne Bacteria

摘要

In foodborne outbreaks, public health administrators must respond promptly and effectively to prevent the spread of pathogens and the recurrence of food poisoning. To do this, it is necessary to identify the causative agents of outbreaks as soon as possible. Traditional culture methods that have been routinely used require several days to identify foodborne bacteria. Traditional culture methods are time-consuming and laborious, since the necessary culture conditions and nutrient media differ from bacteria to bacteria. Thus, simple and reasonable methods for simultaneously detecting various food-poisoning bacterial species are preferable.