Quantitative analysis of #beta#-cryptoxanthin in satsuma mandarin (Citrus unshiu Marc.) juice by C_(30) stationary phase high performance liquid chromatography

摘要

A C_(30) stationary phase high performance liquid chromatographic method was carried out for quantitative determination of #beta#-cryptoxanthin in Satsuma mandarin juice. The studies indicated that #beta#-cryptoxanthin underwent isomerization and degradation on a C_(30) column. The addition of a solvent modifier (ammonium acetate) to the mobile phase improved the recovery of #beta#-cryptoxanthin from the column from ca. 83% to 99%. A thermostatically controlled column (27.5 deg C) demonstrated much better separation of geometric #beta#-cryptoxanthin isomers. Using these methods, #beta#-cryptoxanthin content in Satsuma mandarin juice was found to be 1.01 mg/100g and the two minor peaks of cis-isomers were found on the chromatogram. These two peaks were tentatively identified as 13- and 13'-cis geometric forms of #beta#-cryptoxanthin.