Usefulness of the assay of pig major acute-phase protein (pig-MAP) and polymerase chain reaction (PCR) test for diagnosis of swine toxoplasmosis in meat inspection

摘要

The lymph nodes and sera of pigs with suspected toxoplasmosis during meat inspection at an abattoir were investigated using the PCR for the Blgene of Toxoplasma gondii (PCR test), anti- T. gondii antibody tests (antibody test) and assays for pig majoracute-phase protein (Pig-MAP), haptoglobin (Hp) and alphal-acid glycoprotein (AGP), adding to the microscopic examination of an acridine orange-strained stamp-smear samples from lesions (AOS test). High levels of Pig-MAP in sera were observed in cases that were positive for AOS test, PCR and antibody tests. These findings suggest that the utilizations of Pig-MAP and PCR test provide supplementary information for diagnosis of toxoplasmosis at the time of meat inspection.