DEVELOPMENT AND VALIDATION OF A NEW RP-HPLC METHOD FOR DETERMINATION OF QUERCETIN IN GREEN TEA

摘要

Green tea (Camellia sinensis) contains quercetin as a bioactive compound. Quercetin has anti-inflammatory and anticancer effects. The aim of this paper was to develop and validate an RP-HPLC method for determination of quercetin in green tea simpler and faster than other available methods. RP-HPLC analysis was performed by isocratic elution with a flow rate of 1.0 mL/min. Pure methanol was used as a mobile phase, while the quantification was effected at 370 nm. The separation was performed at 35°C using a C_(18) column (4.6 x 250 mm, 5 urn). The results showed that the peak area response is linear within the concentration range of 10-70 ug/mL (r = 0.9986). The values of LOD and LOQ were 1.2 and 4 ug/mL, respectively. For the intra-day and intra-instrument reproducibility, RSD were in the range of 0.05-0.84% and 0.89—1.55%, respectively. The results of accuracy for the different concentrations of quercetin (40, 50 and 60 ug/mL) were 101.3, 98.4, and 98.2%. The developed and validated method was successfully applied to the determination of quercetin in green tea extract.