DETERMINATION OF SOPHOCARPINE, MATRINE AND SOPHORIDINE IN KLHUANG INJECTION BY GC-MS

摘要

A GC-MS procedure was developed to determine sophocarpine (SC). matrir.e (MT) and sophoridine (SRI) in KUHUANG injection. The chromatographic separation was performed on a HP-5MS column (30 m x 0.35 mm i.d., 0.25 mu m film thickness) with helium as the carrier gas. The oven temperature was programmed from 170 to 205°C at a rate of 0.8°C/min. n-tetracosane was used as the internal standard (I.S.). There was a good linear relationship between the peak area ratio of analyte to I.S. and the concentration of analyte in the ranse 0.0360-0.10 mg/mL for SC. 0.0461-0.346 mg/mL for MT, and 0.0473-0.355 me/mL for SRI. The recoveries were 86.1-100.9% for SC. 87.9-104.0% for MT, and 92.5-107.2% for SRI. The inter-day and intra-day relative standard deviation (RSD)of determinations were less than 4.6. 2.7 and 5.1% for the assav of SC. MT and SRI. respectively. The limits of detection (LODs) were 0.018 mg/rnL for SC. 0.0231 mg/mL for MT. and 0.0287 me/mL for SRI.'The limits of quantitation (LOQs) were 0.036 mg/mL for SC. 0.0461 mg/mL for MT. and 0.0473 mg/mL for SRI. The results indicate that the developed method could be used to improve the quality control of KUHUANG injection.