Race differentiation of Fusarium solani i. sp. cucurbitae by PCR-RFLP

摘要

Differentiations between race 1 and race 2 of Fusarium solani f. sp. cucurbitae by PCR-RFLP analysis of the ITS region including 5.8S rDNA were attempted. Twenty five isolates of F. solani f. sp. cucurbitae race 1, 7 isolates of race 2, 6 isolates ofF. solani which were isolated from imported pumpkin and melon fruits, 10 isolates of 6 for-mae speciales of F. solani and 13 isolates of 7 other species of Fusarium were used. PCR products of 2 races digested with restriction enzymes, Afa I or Hap II, had different RFLP patterns between race 1 and race 2 and there was no difference within the same race. Furthermore, race 1 had different RFLP pattern with other Fusarium spp. Results of this study suggested that this method was able to replace inoculationtest to pumpkin seedling which have been used to differentiate between race 1 and race 2 of F. solani f. sp. cucurbitae.