Promotion of in vitro Shoot Formation from Protocorm-like Bodies of a Hybrid between Tropical and Temperate Cymbidium Species

摘要

Three different treatments, which are known to promote shoot formation in temperate Cymbidium species, were applied to the protocorm-like bodies (PLBs) of Cymbidium Sleeping Beauty > Cymbidium sinense (Andr.) Willd, a hybrid between tropical and temperate Cymbidium species. Shoot formation from the PLBs was promoted on Murashige and Skoog (MS) medium in which NH_4NO_3 and KNO_3 were reduced to 25 and 50%, respectively. The highest shoot formation rate (77%) was obtained on MS medium supplemented with0.1 mg-l~(-1) AgNO_3. The most favorable combination for shoot formation was 10 mg-L~(-1) of 6-benzyladenine (BA) and 0.1 mg-L~(-1)a-naphthaleneacetic acid (NAA).