Inhibitory Effect on Melanin Formation by Marine Bacterial Glycosaminoglycan

摘要

The polysaccharide produced by a marine bacterial strain designated P-21295 was found to be a high molecular weight glycosaminoglycan composed of galactose, galacturonic acid, glucosamine, galactosamine, alanine, and pyruvate, together with N- and 0-acetyl groups. This glycosaminoglycan suppressed the melanin formation in mouse B16F10 cells. The effect was dose dependent without any observed impact on the cell proliferation, indicating that this glycosaminiglycan will be a useful candidate of skin whitening cosmetics. Phenotypic and genetic features of the strain P-21295 suggest that this strain belongs to the Gram-negative Cobetia marina in the class Gammaproteo-bacteria containing the genus Pseudomonas and Vibrio. However, some important phenotypic features of the strain P-21295 differed with those of the type strain C. marina DMS4741.