Phosphoinositide 3-kinase beta, phosphoinositide 3-kinase delta, and phosphoinositide 3-kinase gamma mediate the anti-inflammatory effects of magnesium sulfate

摘要

Background: We previously demonstrated that inhibiting phosphoinositide 3-kinase (PI3K) or activating L-type calcium channels blocked the anti-inflammatory effects of magnesium sulfate (MgSO4). However, the question as which class I PI3K isoform (PI3K alpha, PI3K beta, PI3K delta, or PI3K gamma) is involved in this regard remains unstudied. The question as whether MgSO4 and L-type calcium channels interact to influence PI3K activation also remains unstudied. We therefore designed this study to test two hypotheses: (1) inhibiting PI3K alpha, PI3K beta, PI3K delta, or PI3K gamma would block the anti-inflammatory effects of MgSO4 and (2) activating L-type calcium channels would block the effects of MgSO4 on activating PI3K.