Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan (R) real-time RT-PCR assays for respiratory pathogens

摘要

Tetra methylrhodamine (TAMRA (R)) and black hole quencher 1(BHQ1 (R)) quenched probes and five one-step RT-PCR kits were evaluated in TaqMan (R) real-time RT-PCR assays for detection of respiratory pathogens. The intra-assay variability of the BHQ1 (R) probes were 1.2-2.8-fold lower than those of the TAMRA (R) probes. All kits amplified the specific targets, but differed in their sensitivity by up to 3 orders of magnitude. The AgPath-ID (TM) kit provided the best overall performance for all assay targets