Thermokinetic research method for bacterial growth in conduction calorimeter

摘要

This paper presents the basic equations of thermokinetics and the thermoanalytical curve equation for bacterial growth in conduction calorimeter on the basis of the basic theory of thermokinetics. The bacterial growths in the log phase for Vibro metschnikovii and Bacillus subtilis at different temperatures were calorimetrically investigated. The rate constant of bacterial growth, the cooling constant of the thermokinetic system, the generation time and the pre-exponential factor at different temperature were obtained, which allowed to evaluate the activation energy of bacterial growth (E_a). According to the transition-state theory of chemical kinetics, the activation enthalpy (ΔS~≠), the activation Gibbs free energy (ΔG~≠) and equilibrium constant (K~≠) of the activated state at different temperatures were also obtained. The above results showed that the research method suggested in this paper was reasonable.