Effect of diphenhydramine on myocardial injury caused by organophosphate poisoning

摘要

Background. The aim of this experimental study was to investigate whether diphenhydramine could prevent or diminish myocardial injury caused by organophosphate poisoning as defined by histologic findings and cardiac troponin I (cTnl) levels. Methods. Twenty-four Sprague-Dawley rats were divided into equal three groups. Group 1 did not receive any agent during the experiment. Group 2 received 0.8 g/kg fenthion subcutaneously followed by normal saline (3 ml/kg) intramuscularly 30 minutes later. Group 3 received 0.8 g/kg fenthion subcutaneously, followed by diphenhydramine 30 mg/kg (in 3 ml/kg) intramuscularly 30 minutes later. All rats underwent laparotomy and thoracotomy while under anesthesia at 24 hours. Results. Treatment with diphenhydramine significantly decreased the blood cTnl levels. Additionally, diphenhydramine significantly reduced myocardial injury, including edema, inflammation, vacuolization and necrosis, as determined by pathologic scoring. Conclusion. Organophosphate poisoning can cause myocardial injury as determined by measurement of I cTnl levels. Our study demonstrates that this injury can be attenutated by the administration of diphenydramine.