Towards a standardization of the murine ferric chloride-induced carotid arterial thrombosis model.

摘要

Mouse models of thrombosis have played a vital role in understanding the pathological process of thrombus formation in vivo [1-3]. The most commonly used model is ferric chloride (FeCl_3) injury of the carotid artery. The goal of this report from the Animal Models Subcommittee of the International Society of Thrombosis and Hemostasis is to standardize this model.FeCl_3 is applied to the exterior surface of vessels and triggers vascular wall injury and denudation of the endothelium via a mechanism involving the generation of reactive oxygen species [4,5]. Exposure of collagen triggers platelet activation and exposure of tissue factor activates the coagulation cascade [5,6].