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METABOLIC CONTROL ANALYSIS OF INHIBITORY FEEDBACK INTERACTION - APPLICATION TO BIOTECHNOLOGICAL PROCESSES

机译:抑制反馈相互作用的代谢控制分析-在生物技术过程中的应用

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摘要

Based on a simplified model of a linear metabolic pathway (Kacser & Burns, 1973), and on the principles of Metabolic Control Analysis, an equation useful for predicting the maximum flux through a system after removing inhibitory feedback interaction is presented. The analysis shows that only when the flux control coefficient of the inhibited enzyme is significant (greater than 0.5) is it worthwhile to act on the regulatory features of the feedback interaction. The approach is then applied to a case study and the magnitude of the flux increase and the quality of the prediction studied in two different profiles of flux control coefficients. (C) 1996 Academic Press Limited. [References: 16]
机译:基于线性代谢途径的简化模型(Kacser&Burns,1973),基于代谢控制分析的原理,提出了一个方程,该方程可用于预测去除抑制性反馈相互作用后通过系统的最大通量。分析表明,只有当被抑制的酶的通量控制系数显着(大于0.5)时,才有必要对反馈相互作用的调节特征起作用。然后将该方法应用于案例研究,并在两种不同的磁通控制系数曲线中研究磁通量的增加幅度和预测质量。 (C)1996 Academic Press Limited。 [参考:16]

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