Scutellaria polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast

摘要

BACKGROUNDTo select the antiviral active site of Scutellaria polysaccharide (SPS), safe concentrations of crude total Scutellaria polysaccharide (SPSt) and fractional polysaccharide SPS50, SPS60, SPS70 and SPS80 on chicken embryo fibroblast (CEF) were first compared using the MTT method. Then, SPSt, SPS50, SPS60, SPS70, and SPS80 at five concentrations within the safe concentration, together with Newcastle disease virus (NDV), were added to the cultivating system of CEF in three models: pre-addition of polysaccharide, post-addition of polysaccharide, and simultaneous addition of polysaccharides and NDV after mixing. The effects of SPS on the cellular infectivity of NDV (A(570) value and the highest viral inhibitory rate) were compared using the MTT method. RESULTSAt appropriate concentrations, the five polysaccharides could significantly inhibit the infectivity of NDV on CEF. Among the five polysaccharide groups, the SPS80 group exhibited the highest viral inhibitory rate in the three sample-addition modes. CONCLUSIONThis finding indicates that SPS80 possesses the best efficacy as a component of antiviral polysaccharide drug