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Super-resolved spatial light interference microscopy

机译:超分辨空间光干涉显微镜

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摘要

We report a scheme to achieve resolution beyond the diffraction limit in spatial light interference microscopy (SLIM). By adding a grating to the optical path, the structured illumination technique can be used to improve the resolution by a factor of 2. We show that a direct application of the structured illumination technique, however, has proved to be unsuccessful. Through two crucial modifications, namely, one to the pupil plane of the objective and the other to the demodulation procedure, faithful phase information of the object is recovered and the resolution is improved by a factor of 2.
机译:我们报告了一种方案,以实现超出空间光干涉显微镜(SLIM)中衍射极限的分辨率。通过将光栅添加到光路中,可以使用结构化照明技术将分辨率提高2倍。我们证明,直接应用结构化照明技术已证明是不成功的。通过两个关键的修改,一个修改为物镜的光瞳面,另一修改为解调程序,恢复了对象的真实相位信息,并将分辨率提高了2倍。

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