Developing VUV spectroscopy for protein folding and material luminescence on beamline 4B8 at the Beijing Synchrotron Radiation Facility

摘要

The new 4B8 beamline provides UV–VUV light in the wavelength range from 360 to 120 nm. It uniquely enables two kinds of spectroscopy measurements:synchrotron radiation circular dichroism spectroscopy and VUV excitedfluorescence spectroscopy. The former is mainly used in protein secondarystructure studies, and the latter in VUV excited luminescent materials research.Remote access to fluorescence measurement has been realised and users cancollect data online. Besides steady-state measurements, fluorescence lifetimemeasurements have been established using the time domain method, while alaser-induced temperature jump is under development for protein foldingdynamics using circular dichroism as a probe.