DNA heteropolymorphism of chum salmon detected by denaturing gradient gel electrophoresis and real time PCR

摘要

In order to detect the DNA heteropolymorphism of chum salmon, selected essential genes were examined in different regional chum salmons, i.e., Korean, Japanese and American by denaturing gradient gel electrophoresis (DGGE) and real time PCR methods.From the promoter regions and introns of growth hormone, mtDNA ND1 region, D-loop region, IGF-I, histone H3 and MCH2 several representative primer pairs were obtained and employed for the DGGE with the PCR products from the genomic DNAs of the differentregional chum salmons, mtDNA ND1, D-loop region and IGF-I genes showed marked heteropolymorphism between Korean and American chum salmons. Intron C of growth hormone also showed a heteropolymorphism between Korean and Japanese chum salmons. Whereas heteropolymorphism of histone H3 and MCH2 genes was detected among in Korean, Japanese and American chum salmons in the examined region. The real time PCR disclosed the characteristic incremental production of target DNAs dependent on the heteropolymorphic conditions of genomic DNAs of chum salmons, thus the different regional chum salmons could be grouped by the variable incremental curves. Although the DGGE and real time PCR did not produce the identical results in this study, we suggest that the DGGE andreal time PCR could be used for the primary screening of the DNA heteropolymorphism of different animal genome.