Three strains of Ganoderma tsugae (CCRC36065, CCRC37034, CCRC37038) and three strains of Ganoderma lucidum (CCRC36021, CCRC37029, CCRC37033) were cultivated. Their trierpenoid patterns of the fruit body were analyzed by reverse phase HPLC using a gradient elution of acetonitrile/2% acetic aicd (1/4 and 1/2). The triterpenoid patterns of G. tsugae and G. lucidum are different. But similar 2 and 3 dimensional patterns are obtained among three strains of G. tsugae. Different patterns are found among different strains of G. lucidum. Ganoderic acid A(1), B(2), C(3) and D(4) were isolated from the ethanol extract of G. tsugae.
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