Simultaneous extraction and determination of sulfadiazine and sulfamethoxazole in water samples and aquaculture products using [Bmim]BF_4/(NH_4)_3C_6H_5O_7 aqueous two-phase system coupled with HPLC

摘要

An ionic liquid aqueous two-phase system (ILATPS) of 1-butyl-3- methylimidazolium tetrafluoroborate ([Bmim]BF_4)/ammonium citrate ((NH_4)_3C_6H_5O_7) coupled with high-performance liquid chromatography was developed for the separation and determination of sulfadiazine (SD) and sulfamethoxazole (SMX) in water samples as well as aquaculture products. The effect of such parameters as the types and concentrations of salts, temperature, the concentrations of SD and SMX and the extraction time on the partitioning behavior expressed in terms of extraction efficiency has been evaluated. Under the optimal conditions, this extraction method has been successfully applied to the analysis of SD and SMX in water samples and aquaculture products with the recoveries of 98.29-99.55 % (SD) and 92.09-99.82 % (SMX). The detection limits for two analytes were 0.9 ng mL~(-1) (SD) and 1.8 ng mL~(-1) (SMX). In comparison with the traditional solvent extraction, ILATPS is much simpler and more environmentally friendly for the separation and enrichment of the sulfonamides antibiotics.