Flow Injection Determination of Antibacterial Drug Trimethoprim in Pharmaceutical Preparations with tris(2,2'-Bipyridyl)Ruthenium(III) Chemiluminescence

摘要

In the present study, a simple and sensitive chemiluminescence method using tris(2,2'-bipyridyl)ruthenium(III) with flow injection detection is reported for the analysis of trimethoprim in pharmaceutical preparations. The calibration graph was linear over the concentration range of 0.05-5.0 μg mL~(-1) with relative standard deviations (n = 4) in the range 1.0 - 2.5%. The limit of detection (3σ blank) was 0.008 μg mL~(-1) with a sample throughput of 100 h~(-1). The effect of some organic compounds, common excipients used in pharmaceutical preparations, cations & anions were studied for trimethoprim determination. Ru(bipy)3~(2+) was chemically oxidized to Ru(bipy)3~(3+) online using lead dioxide mini column.