ESTERIFICATION PATTERNS OF LIPASES FOR SYNTHESIZING TRICAPROYLGLYCEROLS IN ORGANIC SOLVENT

摘要

To investigate the synthetic patterns of triglyceride (triacylglycerol) by lipases in organic solvent, esterification patterns of triglyceride, diglyceride, and monoglyceride were monitored at Various reaction times with 10 lipases. As a model study, tricaprin was synthesized from glycerol and capric acids (C-10:0) in isooctane. Lipases that were known to give nonspecific hydrolysis in aqueous solvent, such ac lipase from Candida cylindracea, Lipase OF-360 (from C. rugosa), and Lipase MT (C. rugosa) showed nonspecific synthesis of tricaprin in organic solvent (Group I). There are two groups for esterifying triglycerides in organic solvent with 1,3-specific lipases: one consists of the lipases from Rhizomucor miehei, Pseudomonas aeruginosa (Lipase PS), and Chromobacterium viscosum (Lipase CV) (Group II), and another (Group III) is represented by Lipase AP (Aspergillus niger), Lipase FAP-15 (Rhizopus javanicus), and Lipase D (R. delemar). Although both groups showed 1,3-specific hydrolysis in aqueous solvent, Group III has stricter 1,3-specificity for the synthesis of tricaprin from dicaprin. [References: 18]